Kinetics on Agilent NMR

Kinetics on Agilent NMR

(1) Set experiment as usual:

Login with Yale NetID/password.

“Start” -> “Eject” and put your sample into the magnet.

Left side of VnmrJ, click “Protocol”.

Click “New Study” -> “Sample name” -> “Solvent” -> Select experiments.

Modify default parameters: double click the experiment -> modify the parameters -> “Show time” and “Save” to update.

2) Set kinetics:

Double clicks the experiment again ->

Click “Array” button ->  ‘Enter “pad” as “param name” , here ‘pad’ is pre-acquisition delay, which determines the interval between two adjacent experiments,-> “Array size” is the number of the total experiments -> “Increment” is the time increment of pad, it is zero if the interval between adjust experiments are the same  -> On the right side of the table, double check if the list is correct.

For example, if your kinetics needs acquire a data every 30 minutes, and total 16 experiments, then you can type ‘da’, and hit button “Acquire” -> “Overview”, you should be able see the table like that:

ACQUISITION ARRAY

Array pad

Arraydim       16

i          pad

1        1800

2        1800

3        1800

.

.

16       1800

If you do not want the first experiment has a long waiting time, you can type

Pad[1]= 1.

After entering array parameters, hit “Save” to update.

If you need accurate timing, the times spend for data acquisition should be taken into account.   For example, “Show time” gives 2 minutes and 30 seconds for individual experiment.  Then ‘pad’ should be modified as (1800-150)=1650.

3) After the setup, hit “submit” to start.

4) The data will be saved automatically.  

5) When an arrayed folder is dragged and dropped into Mnova, Mnova proceeds to process all the individual spectra, one after the other along the only valid dimension (F2) and display them as a stacked data set.