All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube
A400a
Mass |
Concentration |
Proton |
Carbon |
gCOSY |
gHSQC |
gHMBC |
0.03 mg |
0.1 mM |
10 h 40 min |
35389 hours |
1785 hours |
1886 hours |
7129 hours |
0.06 mg |
0.2 mM |
2 h 40 min |
10516 hours |
46 h 28 min |
474 h 12 min |
1785 hours |
0.15 mg |
0.5 mM |
0 h 26 min |
1415 h 33 min |
6 h 10 min |
75 h 01 min |
284 h 44 min |
0.3 mg |
1 mM |
0 h 06 min |
354 h 01 min |
1 h 40 min |
18 h 53 min |
71 h 19 min |
0.6 mg |
2 mM |
0 h 01 min |
88 h 31 min |
0 h 26 min |
4 h 42 min |
17 h 51 min |
1.5 mg |
5 mM |
< 1 min |
14 h 09 min |
0 h 03 min |
0 h 45 min |
2 h 51 min |
3 mg |
10 mM |
< 1 min |
3 h 32 min |
0 h 03 min |
0 h 16 min |
1 h 07 min |
6 mg |
20 mM |
< 1 min |
0 h 53 min |
0 h 03 min |
0 h 08 min |
0 h 17 min |
12 mg |
40 mM |
< 1 min |
0 h 13 min |
0 h 03 min |
0 h 08 min |
0 h 17 min |
15 mg |
50 mM |
< 1 min |
0 h 08 min |
0 h 03 min |
0 h 08 min |
0 h 17 min |
> 30 mg |
100+ mM |
< 1 min |
0 h 04 min |
0 h 03 min |
0 h 08 min |
0 h 17 min |
A400b
Mass |
Concentration |
Proton |
Carbon |
gCOSY |
gHSQC |
gHMBC |
0.03 mg |
0.1 mM |
44 h 44 min |
50960 hours |
644 hours |
7862 hours |
29844 hours |
0.06 mg |
0.2 mM |
11 h 11 min |
12740 hours |
161 hours |
1965 hours |
7461 hours |
0.15 mg |
0.5 mM |
1 h 47 min |
2038 h 24 min |
25 h 47 min |
319 h 18 min |
1193 h 57 min |
0.3 mg |
1 mM |
0 h 27 min |
509 h 36 min |
6 h 30 min |
78 h 37 min |
298 h 40 min |
0.6 mg |
2 mM |
0 h 07 min |
127 h 27 min |
1 h 38 min |
19 h 48 min |
75 h 40 min |
1.5 mg |
5 mM |
0 h 01 min |
20 h 23 min |
0 h 16 min |
3 h 08 min |
12 h 15 min |
3 mg |
10 mM |
< 1 min |
5 h 15 min |
0 h 06 min |
0 h 47 min |
1 h 32 min |
6 mg |
20 mM |
< 1 min |
1 h 16 min |
0 h 03 min |
0 h 08 min |
0 h 17 min |
12 mg |
40 mM |
< 1 min |
0 h 19 min |
0 h 03 min |
0 h 08 min |
0 h 17 min |
15 mg |
50 mM |
< 1 min |
0 h 12 min |
0 h 03 min |
0 h 08 min |
0 h 17 min |
> 30 mg |
100+ mM |
< 1 min |
0 h 04 min |
0 h 03 min |
0 h 08 min |
0 h 17 min |
A400c
Mass |
Concentration |
Proton |
Carbon |
gCOSY |
gHSQC |
gHMBC |
0.03 mg |
0.1 mM |
10 h 40 min |
35389 hours |
1785 hours |
1886 hours |
7129 hours |
0.06 mg |
0.2 mM |
2 h 40 min |
10516 hours |
46 h 28 min |
474 h 12 min |
1785 hours |
0.15 mg |
0.5 mM |
0 h 26 min |
1415 h 33 min |
6 h 10 min |
75 h 01 min |
284 h 44 min |
0.3 mg |
1 mM |
0 h 06 min |
354 h 01 min |
1 h 40 min |
18 h 53 min |
71 h 19 min |
0.6 mg |
2 mM |
0 h 01 min |
88 h 31 min |
0 h 26 min |
4 h 42 min |
17 h 51 min |
1.5 mg |
5 mM |
< 1 min |
14 h 09 min |
0 h 03 min |
0 h 45 min |
2 h 51 min |
3 mg |
10 mM |
< 1 min |
3 h 32 min |
0 h 03 min |
0 h 16 min |
1 h 07 min |
6 mg |
20 mM |
< 1 min |
0 h 53 min |
0 h 03 min |
0 h 08 min |
0 h 17 min |
12 mg |
40 mM |
< 1 min |
0 h 13 min |
0 h 03 min |
0 h 08 min |
0 h 17 min |
15 mg |
50 mM |
< 1 min |
0 h 08 min |
0 h 03 min |
0 h 08 min |
0 h 17 min |
> 30 mg |
100+ mM |
< 1 min |
0 h 04 min |
0 h 03 min |
0 h 08 min |
0 h 17 min |
A500a
Mass |
Concentration |
Proton |
Carbon |
gCOSY |
gHSQC |
gHMBC |
0.03 mg |
0.1 mM |
5 hours |
16962 hours |
67 h 16 min |
820 h 42 min |
3113 h 37 min |
0.06 mg |
0.2 mM |
1 h 10 min |
424 h 02 min |
16 h 50 min |
205 h 15 min |
779 h 06 min |
0.15 mg |
0.5 mM |
0 h 11 min |
678 h 28 min |
2 h 45 min |
32 h 50 min |
124 h 34 min |
0.3 mg |
1 mM |
0 h 03 min |
170 h 00 min |
0 h 42 min |
8 h 20 min |
31 h 09 min |
0.6 mg |
2 mM |
< 1 min |
4 h 15 min |
0 h 11 min |
2 h 05 min |
7 h 48 min |
1.5 mg |
5 mM |
< 1 min |
3 h 11 min |
0 h 03 min |
0 h 08 min |
1 h 40 min |
3 mg |
10 mM |
< 1 min |
1 h 42 min |
0 h 03 min |
0 h 08 min |
0 h 17 min |
6 mg |
20 mM |
< 1 min |
0 h 25 min |
0 h 03 min |
0 h 08 min |
0 h 17 min |
12 mg |
40 mM |
< 1 min |
0 h 06 min |
0 h 03 min |
0 h 08 min |
0 h 17 min |
15 mg |
50 mM |
< 1 min |
0 h 04 min |
0 h 03 min |
0 h 08 min |
0 h 17 min |
> 30 mg |
100+ mM |
< 1 min |
0 h 04 min |
0 h 03 min |
0 h 08 min |
0 h 17 min |
A500b
Mass |
Concentration |
Proton |
Carbon |
gCOSY |
gHSQC |
gHMBC |
0.03 mg |
0.1 mM |
5 hours |
16962 hours |
67 h 16 min |
820 h 42 min |
3113 h 37 min |
0.06 mg |
0.2 mM |
1 h 10 min |
424 h 02 min |
16 h 50 min |
205 h 15 min |
779 h 06 min |
0.15 mg |
0.5 mM |
0 h 11 min |
678 h 28 min |
2 h 45 min |
32 h 50 min |
124 h 34 min |
0.3 mg |
1 mM |
0 h 03 min |
170 h 00 min |
0 h 42 min |
8 h 20 min |
31 h 09 min |
0.6 mg |
2 mM |
< 1 min |
4 h 15 min |
0 h 11 min |
2 h 05 min |
7 h 48 min |
1.5 mg |
5 mM |
< 1 min |
3 h 11 min |
0 h 03 min |
0 h 08 min |
1 h 40 min |
3 mg |
10 mM |
< 1 min |
1 h 42 min |
0 h 03 min |
0 h 08 min |
0 h 17 min |
6 mg |
20 mM |
< 1 min |
0 h 25 min |
0 h 03 min |
0 h 08 min |
0 h 17 min |
12 mg |
40 mM |
< 1 min |
0 h 06 min |
0 h 03 min |
0 h 08 min |
0 h 17 min |
15 mg |
50 mM |
< 1 min |
0 h 04 min |
0 h 03 min |
0 h 08 min |
0 h 17 min |
> 30 mg |
100+ mM |
< 1 min |
0 h 04 min |
0 h 03 min |
0 h 08 min |
0 h 17 min |
A600a
Mass |
Concentration |
Proton |
Carbon |
gCOSY |
gHSQC |
gHMBC |
0.03 mg |
0.1 mM |
0 h 24 min |
1298 hours |
5 h 54 min |
72 h 04 min |
272 h 22 min |
0.06 mg |
0.2 mM |
0 h 06 min |
324 h 32 min |
1 h 28 min |
19 h 35 min |
68 h 32 min |
0.15 mg |
0.5 mM |
0 h 01 min |
51 h 56 min |
0 h 16 min |
3 h 11 min |
11 h 10 min |
0.3 mg |
1 mM |
< 1 min |
13 h 00 min |
0 h 03 min |
0 h 47 min |
2 h 47 min |
0.6 mg |
2 mM |
< 1 min |
3 h 15 min |
0 h 03 min |
0 h 16 min |
1 h 07 min |
1.5 mg |
5 mM |
< 1 min |
0 h 31 min |
0 h 03 min |
0 h 08 min |
0 h 34 min |
3 mg |
10 mM |
< 1 min |
0 h 08 min |
0 h 03 min |
0 h 08 min |
0 h 17 min |
6 mg |
20 mM |
< 1 min |
0 h 04 min |
0 h 03 min |
0 h 08 min |
0 h 17 min |
12 mg |
40 mM |
< 1 min |
0 h 04 min |
0 h 03 min |
0 h 08 min |
0 h 17 min |
15 mg |
50 mM |
< 1 min |
0 h 04 min |
0 h 03 min |
0 h 08 min |
0 h 17 min |
> 30 mg |
100+ mM |
< 1 min |
0 h 04 min |
0 h 03 min |
0 h 08 min |
0 h 17 min |
All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube