Short Name:
A400b
Instrument Type:
NMR & EPR
Location:
KCL 3
Date of Acquisition:
October 2013
Description:
2 channel system. AutoX DB probe installed. Variable-temperature and H/F experiments available.
Guides and Standard Operating Procedures
Best experiments:
- PROTON
- Simultaneous F19 and H1 experiments (i.e. F19 with H1 decoupling)
- Variable Temperature experiments (with appropriate CBIC training)
- Kinetics experiments
Acceptable experiments:
- C13CPD
- 2D HSQC and HMBC experiments
- 1D/2D NOE experiments
Not available:
- Nuclei other than H1, F19 & C13
Time Required for NMR Experiments on A400b:
Mass | Concentration | Proton | Carbon | gCOSY | gHSQC | gHMBC |
0.03 mg | 0.1 mM | 44 h 44 min | 50960 hours | 644 hours | 7862 hours | 29844 hours |
0.06 mg | 0.2 mM | 11 h 11 min | 12740 hours | 161 hours | 1965 hours | 7461 hours |
0.15 mg | 0.5 mM | 1 h 47 min | 2038 h 24 min | 25 h 47 min | 319 h 18 min | 1193 h 57 min |
0.3 mg | 1 mM | 0 h 27 min | 509 h 36 min | 6 h 30 min | 78 h 37 min | 298 h 40 min |
0.6 mg | 2 mM | 0 h 07 min | 127 h 27 min | 1 h 38 min | 19 h 48 min | 75 h 40 min |
1.5 mg | 5 mM | 0 h 01 min | 20 h 23 min | 0 h 16 min | 3 h 08 min | 12 h 15 min |
3 mg | 10 mM | < 1 min | 5 h 15 min | 0 h 06 min | 0 h 47 min | 1 h 32 min |
6 mg | 20 mM | < 1 min | 1 h 16 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
12 mg | 40 mM | < 1 min | 0 h 19 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
15 mg | 50 mM | < 1 min | 0 h 12 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
> 30 mg | 100+ mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube