Disclaimer: All information on this site is subject to change and the actual experimental methods used might differ from the ones found here!
Recommendations:
MS_Sample_Method_Recommendations_v2.docx
Method Descriptions (methods might have changed over time, please double-check!):
Shimadzu 9030 HRMS Description
Thermo Fisher QExactive Orbitrap HRMS Description
Software:
Data exchange
MS data can be exported as mzXML or mzML files and shared via box or google drive upon request. The files contain TIC and MS data, EIC can be extracted through user analysis. These data files can be opened with MestreNova (updated and fully installed licenses necessary!):
Download the software and license bundle from the CBIC homepage:
https://cbic.yale.edu/yale-only/mnova-download
Close MestreNova (for update of existing installation), and run as Administrator by:
Windows Start menu -> MestreNova Folder -> Right Click on MestreNova -> more -> Run as Administrator
Install the Licenses again, close the software and start it as a normal user.
Complex data workups and simple txt data can be provided based on the project.
Software for opening and converting ThermoFisher .raw files: http://msfragger.nesvilab.org/tutorial_convert.html
Acknowledgements:
If you use CBIC instruments in your research, we request that you acknowledge our facility in your written publications or grant applications as follows:
Acknowledgement: This research made use of the Chemical and Biophysical Instrumentation Center at Yale University (RRID:SCR_021738). Equipment was purchased with funds from Yale University.
Optional for Orbitrap QExactive MS: NSF MRI grant CHE-1828190.