A400b Agilent DD2 400 MHz NMR Spectrometer (Manual)

Short Name: 
A400b
Instrument Type: 
NMR & EPR
Location: 
KCL 3
Date of Acquisition: 
October 2013
Description: 

2 channel system. AutoX DB probe installed.  Variable-temperature and H/F experiments available.

Best experiments:

  • PROTON
  • Simultaneous F19 and H1 experiments (i.e. F19 with H1 decoupling)
  • Variable Temperature experiments (with appropriate CBIC training)
  • Kinetics experiments

Acceptable experiments:

  • C13CPD
  • 2D HSQC and HMBC experiments
  • 1D/2D NOE experiments

Not available:

  • Nuclei other than H1, F19 & C13

Time Required for NMR Experiments on A400b:

Mass Concentration Proton Carbon gCOSY  gHSQC gHMBC
0.03 mg 0.1 mM 44 h 44 min 50960 hours 644 hours 7862 hours 29844 hours
0.06 mg 0.2 mM 11 h 11 min 12740 hours 161 hours 1965 hours 7461 hours
0.15 mg 0.5 mM 1 h 47 min 2038 h 24 min 25 h 47 min 319 h 18 min 1193 h 57 min
0.3 mg 1 mM 0 h 27 min 509 h 36 min 6 h 30 min 78 h 37 min 298 h 40 min
0.6 mg 2 mM 0 h 07 min 127 h 27 min 1 h 38 min 19 h 48 min 75 h 40 min
1.5 mg 5 mM 0 h 01 min 20 h 23 min 0 h 16 min 3 h 08 min 12 h 15 min
3 mg 10 mM < 1 min 5 h 15 min 0 h 06 min 0 h 47 min 1 h 32 min
6 mg 20 mM < 1 min 1 h 16 min 0 h 03 min 0 h 08 min 0 h 17 min
12 mg 40 mM < 1 min 0 h 19 min 0 h 03 min 0 h 08 min 0 h 17 min
15 mg 50 mM < 1 min 0 h 12 min 0 h 03 min 0 h 08 min 0 h 17 min
> 30 mg 100+ mM < 1 min 0 h 04 min 0 h 03 min 0 h 08 min 0 h 17 min

All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube

Instrument Image: