Instrument Catalog

A400a Agilent DD2 400 MHz NMR Spectrometer (Autosampler)
Short Name: A400a
Instrument Type: NMR & EPR
Instrument Location: KCL 3
Date of Acquisition: August 2013

2 channel system with AS7620 96-sample changer. OneNMR
probe with auto-tune-and-match installed.

Undergraduates have priority on this instrument during lab periods.

Best experiments:

  • PROTON
  • COSY
  • Other nuclei: F19 (no H1 decoupling), P31, B11, (others by request)

Acceptable experiments:

  • C13CPD (night time)
  • 2D HSQC and HMBC experiments
  • 1D/2D NOE experiments

Not available:

  • Variable Temperature experiments
  • Kinetics experiments
  • Simultaneous F19 and H1 experiments

Time Required for NMR Experiments on A400a:

Mass Concentration Proton Carbon gCOSY  gHSQC gHMBC
0.03 mg 0.1 mM 10 h 40 min 35389 hours 1785 hours 1886 hours 7129 hours
0.06 mg 0.2 mM 2 h 40 min 10516 hours 46 h 28 min 474 h 12 min 1785 hours
0.15 mg 0.5 mM 0 h 26 min 1415 h 33 min 6 h 10 min 75 h 01 min 284 h 44 min
0.3 mg 1 mM 0 h 06 min 354 h 01 min 1 h 40 min 18 h 53 min 71 h 19 min
0.6 mg 2 mM 0 h 01 min 88 h 31 min 0 h 26 min 4 h 42 min 17 h 51 min
1.5 mg 5 mM < 1 min 14 h 09 min 0 h 03 min 0 h 45 min 2 h 51 min
3 mg 10 mM < 1 min 3 h 32 min 0 h 03 min 0 h 16 min 1 h 07 min
6 mg 20 mM < 1 min 0 h 53 min 0 h 03 min 0 h 08 min 0 h 17 min
12 mg 40 mM < 1 min 0 h 13 min 0 h 03 min 0 h 08 min 0 h 17 min
15 mg 50 mM < 1 min 0 h 08 min 0 h 03 min 0 h 08 min 0 h 17 min
> 30 mg 100+ mM < 1 min 0 h 04 min 0 h 03 min 0 h 08 min 0 h 17 min

All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube

A400b Agilent DD2 400 MHz NMR Spectrometer (Manual)
Short Name: A400b
Instrument Type: NMR & EPR
Instrument Location: KCL 3
Date of Acquisition: October 2013

2 channel system. AutoX DB probe installed.  Variable-temperature and H/F experiments available.

Best experiments:

  • PROTON
  • Simultaneous F19 and H1 experiments (i.e. F19 with H1 decoupling)
  • Variable Temperature experiments (with appropriate CBIC training)
  • Kinetics experiments

Acceptable experiments:

  • C13CPD
  • 2D HSQC and HMBC experiments
  • 1D/2D NOE experiments

Not available:

  • Nuclei other than H1, F19 & C13

Time Required for NMR Experiments on A400b:

Mass Concentration Proton Carbon gCOSY  gHSQC gHMBC
0.03 mg 0.1 mM 44 h 44 min 50960 hours 644 hours 7862 hours 29844 hours
0.06 mg 0.2 mM 11 h 11 min 12740 hours 161 hours 1965 hours 7461 hours
0.15 mg 0.5 mM 1 h 47 min 2038 h 24 min 25 h 47 min 319 h 18 min 1193 h 57 min
0.3 mg 1 mM 0 h 27 min 509 h 36 min 6 h 30 min 78 h 37 min 298 h 40 min
0.6 mg 2 mM 0 h 07 min 127 h 27 min 1 h 38 min 19 h 48 min 75 h 40 min
1.5 mg 5 mM 0 h 01 min 20 h 23 min 0 h 16 min 3 h 08 min 12 h 15 min
3 mg 10 mM < 1 min 5 h 15 min 0 h 06 min 0 h 47 min 1 h 32 min
6 mg 20 mM < 1 min 1 h 16 min 0 h 03 min 0 h 08 min 0 h 17 min
12 mg 40 mM < 1 min 0 h 19 min 0 h 03 min 0 h 08 min 0 h 17 min
15 mg 50 mM < 1 min 0 h 12 min 0 h 03 min 0 h 08 min 0 h 17 min
> 30 mg 100+ mM < 1 min 0 h 04 min 0 h 03 min 0 h 08 min 0 h 17 min

All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube

A400c Agilent DD2 400 MHz NMR Spectrometer (Autosampler)
Short Name: A400c
Instrument Type: NMR & EPR
Instrument Location: KCL 3
Date of Acquisition: October 2013

2 channel system with AS7620 96-sample changer. OneNMR probe with auto-tune-and-match installed.

Primarily used for short, routine NMR experiments where data is needed quickly.

Best experiments:

  • PROTON 
  • COSY
  • Other nuclei: F19 (no H1 decoupling), P31, B11, (others by request)

Acceptable experiments:

  • C13CPD (night time)
  • 2D HSQC and HMBC experiments (night time)
  • 1D/2D NOE experiments (night time)

Not available:

  • Variable Temperature experiments
  • Kinetics experiments
  • Simultaneous F19 and H1 experiments

Time Required for NMR Experiments on A400c:

Mass Concentration Proton Carbon gCOSY  gHSQC gHMBC
0.03 mg 0.1 mM 10 h 40 min 35389 hours 1785 hours 1886 hours 7129 hours
0.06 mg 0.2 mM 2 h 40 min 10516 hours 46 h 28 min 474 h 12 min 1785 hours
0.15 mg 0.5 mM 0 h 26 min 1415 h 33 min 6 h 10 min 75 h 01 min 284 h 44 min
0.3 mg 1 mM 0 h 06 min 354 h 01 min 1 h 40 min 18 h 53 min 71 h 19 min
0.6 mg 2 mM 0 h 01 min 88 h 31 min 0 h 26 min 4 h 42 min 17 h 51 min
1.5 mg 5 mM < 1 min 14 h 09 min 0 h 03 min 0 h 45 min 2 h 51 min
3 mg 10 mM < 1 min 3 h 32 min 0 h 03 min 0 h 16 min 1 h 07 min
6 mg 20 mM < 1 min 0 h 53 min 0 h 03 min 0 h 08 min 0 h 17 min
12 mg 40 mM < 1 min 0 h 13 min 0 h 03 min 0 h 08 min 0 h 17 min
15 mg 50 mM < 1 min 0 h 08 min 0 h 03 min 0 h 08 min 0 h 17 min
> 30 mg 100+ mM < 1 min 0 h 04 min 0 h 03 min 0 h 08 min 0 h 17 min

All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube

A500a Agilent DD2 500 MHz NMR Spectrometer (Manual)
Short Name: A500a
Instrument Type: NMR & EPR
Instrument Location: KCL 3
Date of Acquisition: January 2014

2 channel system, OneNMR probe with auto-tune-and-match installed.

Best experiments:

  • PROTON
  • COSY
  • 2D HSQC and HMBC experiments
  • 1D/2D NOE experiments
  • Other nuclei: F19 (no H1 decoupling), P31, B11, (others by request)
  • Variable Temperature experiments
  • Kinetics experiments

Acceptable experiments:

  • C13CPD

Not available:

  • Simultaneous F19 and H1 experiments

Time Required for NMR Experiments on A500a:

Mass Concentration Proton Carbon gCOSY  gHSQC gHMBC
0.03 mg 0.1 mM 5 hours 16962 hours 67 h 16 min 820 h 42 min 3113 h 37 min
0.06 mg 0.2 mM 1 h 10 min 424 h 02 min 16 h 50 min 205 h 15 min 779 h 06 min
0.15 mg 0.5 mM 0 h 11 min 678 h 28 min 2 h 45 min 32 h 50 min 124 h 34 min
0.3 mg 1 mM 0 h 03 min 170 h 00 min 0 h 42 min 8 h 20 min 31 h 09 min
0.6 mg 2 mM < 1 min 4 h 15 min 0 h 11 min 2 h 05 min 7 h 48 min
1.5 mg 5 mM < 1 min 3 h 11 min 0 h 03 min 0 h 08 min 1 h 40 min
3 mg 10 mM < 1 min 1 h 42 min 0 h 03 min 0 h 08 min 0 h 17 min
6 mg 20 mM < 1 min 0 h 25 min 0 h 03 min 0 h 08 min 0 h 17 min
12 mg 40 mM < 1 min 0 h 06 min 0 h 03 min 0 h 08 min 0 h 17 min
15 mg 50 mM < 1 min 0 h 04 min 0 h 03 min 0 h 08 min 0 h 17 min
> 30 mg 100+ mM < 1 min 0 h 04 min 0 h 03 min 0 h 08 min 0 h 17 min

All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube

A500b Agilent DD2 500 MHz NMR Spectrometer (Manual)
Short Name: A500b
Instrument Type: NMR & EPR
Instrument Location: KCL 3
Date of Acquisition: September 2012

2 channel system, OneNMR probe with auto-tune-and-match installed.

Best experiments:

  • PROTON
  • COSY
  • 2D HSQC and HMBC experiments
  • 1D/2D NOE experiments
  • Other nuclei: F19 (no H1 decoupling), P31, B11, (others by request)
  • Variable Temperature experiments
  • Kinetics experiments

Acceptable experiments:

  • C13CPD

Not available:

  • Simultaneous F19 and H1 experiments

Time Required for NMR Experiments on A500b:

Mass Concentration Proton Carbon gCOSY  gHSQC gHMBC
0.03 mg 0.1 mM 5 hours 16962 hours 67 h 16 min 820 h 42 min 3113 h 37 min
0.06 mg 0.2 mM 1 h 10 min 424 h 02 min 16 h 50 min 205 h 15 min 779 h 06 min
0.15 mg 0.5 mM 0 h 11 min 678 h 28 min 2 h 45 min 32 h 50 min 124 h 34 min
0.3 mg 1 mM 0 h 03 min 170 h 00 min 0 h 42 min 8 h 20 min 31 h 09 min
0.6 mg 2 mM < 1 min 4 h 15 min 0 h 11 min 2 h 05 min 7 h 48 min
1.5 mg 5 mM < 1 min 3 h 11 min 0 h 03 min 0 h 08 min 1 h 40 min
3 mg 10 mM < 1 min 1 h 42 min 0 h 03 min 0 h 08 min 0 h 17 min
6 mg 20 mM < 1 min 0 h 25 min 0 h 03 min 0 h 08 min 0 h 17 min
12 mg 40 mM < 1 min 0 h 06 min 0 h 03 min 0 h 08 min 0 h 17 min
15 mg 50 mM < 1 min 0 h 04 min 0 h 03 min 0 h 08 min 0 h 17 min
> 30 mg 100+ mM < 1 min 0 h 04 min 0 h 03 min 0 h 08 min 0 h 17 min

All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube

A600a Agilent DD2 600 MHz NMR Spectrometer (Autosampler)
Short Name: A600a
Instrument Type: NMR & EPR
Instrument Location: KCL 3
Date of Acquisition: October 2013

3 channel system with AS7510 12-sample automatic sample changer. C{H} cold probe with auto-tune-and-match installed. Highest sensitivity for C13 NMR experiments.

Best experiments:

  • CARBON
  • PROTON

Acceptable experiments:

  • 1D/2D NOE experiments

Not available:

  • Variable Temperature experiments
  • Kinetics experiments
  • Nuclei other than H1 and C13

Time Required for NMR Experiments on A600a:

Mass Concentration Proton Carbon gCOSY  gHSQC gHMBC
0.03 mg 0.1 mM 0 h 24 min 1298 hours 5 h 54 min 72 h 04 min 272 h 22 min
0.06 mg 0.2 mM 0 h 06 min 324 h 32 min 1 h 28 min 19 h 35 min 68 h 32 min
0.15 mg 0.5 mM 0 h 01 min 51 h 56 min 0 h 16 min 3 h 11 min 11 h 10 min
0.3 mg 1 mM < 1 min 13 h 00 min 0 h 03 min 0 h 47 min 2 h 47 min
0.6 mg 2 mM < 1 min 3 h 15 min 0 h 03 min 0 h 16 min 1 h 07 min
1.5 mg 5 mM < 1 min 0 h 31 min 0 h 03 min 0 h 08 min 0 h 34 min
3 mg 10 mM < 1 min 0 h 08 min 0 h 03 min 0 h 08 min 0 h 17 min
6 mg 20 mM < 1 min 0 h 04 min 0 h 03 min 0 h 08 min 0 h 17 min
12 mg 40 mM < 1 min 0 h 04 min 0 h 03 min 0 h 08 min 0 h 17 min
15 mg 50 mM < 1 min 0 h 04 min 0 h 03 min 0 h 08 min 0 h 17 min
> 30 mg 100+ mM < 1 min 0 h 04 min 0 h 03 min 0 h 08 min 0 h 17 min

All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube

A800 Agilent 800 MHz NMR Spectrometer (Manual)
Short Name: A800
Instrument Type: NMR & EPR
Instrument Location: CRB 100
Date of Acquisition: July 2013

4 channel system. Inverse-detect HCN probe, Dual Band (direct-detect) probe, and H{CN} C13-sensitivity enhanced salt-tolerant cold probe available.

Experienced Users Only. Contact CBIC staff for more information.

Analytical Ultracentrifuge Beckman Coulter Proteomelab XL-I
Short Name: AU
Instrument Type: Solution Biophysics
Instrument Location: KCL 3
Date of Acquisition: November 2009

Analytical ultracentrifugation is a column-free separation technique that measures the relative change in the distribution of molecular weights, providing a way to measure heterogeneity, stoichiometry and self-association of proteins, oligomers, aggregates, particles, colloids, and small structures in solution. 

Applied Photophysics Chirascan Circular Dichroism Spectrometer
Short Name: CD
Instrument Type: Solution Biophysics
Instrument Location: KCL 3
Date of Acquisition: September 2013

Chirascan is a modern, high performance circular dichroism (CD) spectrometer operating in the ultraviolet and visible wavelength range. It is typically used to probe the secondary structure or conformation of bio-molecules.

ASI React IR Reaction Monitoring System
Short Name: ReactIR
Instrument Type: Optical/Mass Spec
Instrument Location: KCL 3
Date of Acquisition: June 1998

FTIR for real-time monitoring of chemical reactions in situ.

DLS Dynamic Light Scattering Wyatt DynaPro PlateReader II
Short Name: DLS
Instrument Type: Solution Biophysics
Instrument Location: KCL 3
Date of Acquisition: October 2013

Dynamic light scattering measures the diffusion rate of molecules or particles in solution by observing the fluctuations in scattered light from a laser passing through a sample. The sensitivity of DLS increases with increasing molecular size, with a typical lower size limit of 0.5 nanometers and upper size limit of 10 micrometers.

Typical applications for DLS include:

  • Screening macromolecules prior to crystallization trials
  • Measuring the stability of protein samples for NMR analysis
  • Monitoring molecular aggregation
  • Characterizing nanoparticles

The DynaPro PlateReader II is set up to make these measurements in 96, 384, or 1536 well plates using as little as 5 microliters of sample.

DSC Microcal VP Differential Scanning Calorimeter
Short Name: DSC
Instrument Type: Solution Biophysics
Instrument Location: KCL 3
Date of Acquisition: January 2008

The Microcal VP DSC can characterize the stability of a biomolecule directly in its native form by measuring the heat change associated with its thermal denaturation when heated at a constant rate.

EPR Bruker EleXsys EPR Spectrometer
Short Name: EPR
Instrument Type: NMR & EPR
Instrument Location: KCL 12
Date of Acquisition: September 2000

X-Band Electron Paramagnetic Resonance. Cold Probe (helium temperature) available.

FTIR/Raman Thermo Nicolet 6700
Short Name: FTIR
Instrument Type: Optical/Mass Spec
Instrument Location: KCL 3
Date of Acquisition: November 2006

Diamond ATR cell normally installed. Transmission, DRIFTS, ZnSe ATR, and high-temperature accessories available. Raman with IR-laser source also available.

GC-MS Agilent 6890N/5973
Short Name: GC-MS
Instrument Type: Optical/Mass Spec
Instrument Location: KCL 3
Date of Acquisition: February 2004

Open access GC-MS instrument with 100-position sample changer and 20 meter column installed.

LC-MS Waters ZQ 4000 Single Quad Mass Spectrometer
Short Name: LC-MS
Instrument Type: Optical/Mass Spec
Instrument Location: KCL 3
Date of Acquisition: June 2002

Waters LC system. DART, ESI, APCI sources available.

MALDI-TOF Voyager DE Pro
Short Name: MALDI
Instrument Type: Optical/Mass Spec
Instrument Location: KCL 3
Date of Acquisition: October 2003

MALDI-TOF mass spectrometer with positive and negative modes, linear and reflection-mode (for enhanced resolution) detection.

MAR Image Plate on 007HF+ X-ray Source
Short Name: 007-MAR
Instrument Type: X-ray Diffraction
Instrument Location: KCL 7
Date of Acquisition: October 2009

The MAR image plate detector on our Rigaku 007 HF+ rotating anode X-ray source is suitable for solving protein structures by single-crystal X-ray diffraction.  This instrument can also be used for powder X-ray diffraction on small samples in sealed capillaries.

P-XRD Bruker D8 Focus Powder X-ray Diffractometer
Short Name: PXRD
Instrument Type: X-ray Diffraction
Instrument Location: KCL 7
Date of Acquisition: December 2004

This powder diffractometer can characterize crystallinity, crystal phases, and, in many cases, the identity of solid samples. Our instrument has a sealed-tube copper X-ray source, a scintillation counter with high dynamic range, and Bragg-Brentano geometry with slits providing high resolution for flat powder samples.

Polarimeter Perkin Elmer 341
Short Name: Polarimeter
Instrument Type: Optical/Mass Spec
Instrument Location: KCL 3
Date of Acquisition: January 1997

General-purpose precision Polarimeter with Na and Hg source lamps and Glan-Taylor polarizer.

Rigaku CCD Detector on 007HF+ X-ray Source
Short Name: 007
Instrument Type: X-ray Diffraction
Instrument Location: KCL 7
Date of Acquisition: October 2009

The Saturn 944+ CCD detector on our Rigaku 007 HF+ rotating anode X-ray source is suitable for solving small-molecule and protein structures by single-crystal X-ray diffraction.  This instrument can also be used to screen crystals before sending them to an x-ray beam line at a synchrotron.

Rigaku R-AXIS SPIDER X-ray Diffractometer
Short Name: Spider
Instrument Type: X-ray Diffraction
Instrument Location: KCL 7
Date of Acquisition: October 2009

Copper and Molybdenum X-ray sources are available. The curved image plate detector allows high dynamic range and collection out to a 2Θ angle of 144 degrees in a single image to yield high-resolution single-crystal data.

Rigaku SCXmini Benchtop X-ray Diffractometer
Short Name: Mini
Instrument Type: X-ray Diffraction
Instrument Location: KCL 7
Date of Acquisition: October 2009

The SCXmini is a fully functional benchtop single-crystal X-ray diffractometer with a CCD detector. Students can be trained to collect their own XRD data with this instrument.

TA NanoITC
Short Name: ITC
Instrument Type: Solution Biophysics
Instrument Location: KCL 3
Date of Acquisition: August 2014

By determining heat changes associated with binding between any two biomolecules, ITC measures all binding parameters in a single label-free, in-solution experiment.

TCSPC TD-Fluor Horiba Fluorolog 3 Time Domain Fluorimeter
Short Name: TCSPC
Instrument Type: Solution Biophysics
Instrument Location: KCL 3
Date of Acquisition: December 2009

The Florolog 3 is a modular fluorimeter that can be set up to make time-domain fluorescence measurements at specific wavelengths, or steady-state fluorescence measurements over a range of excitation and/or emission wavelengths. 

TTP Lab-tech Nanoliter Pipetting System
Short Name: Mosquito
Instrument Type: X-ray Diffraction
Instrument Location: KCL 7
Date of Acquisition: October 2009

TTP Labtech’s mosquito allows for the creation of protein crystallization screens with several multi-component drops per well, even in 96-well hanging drop set-ups.

UV-VIS Cary 3E Spectrophotometer
Short Name: UV-VIS
Instrument Type: Optical/Mass Spec
Instrument Location: KCL 3
Date of Acquisition: November 1995

Standard cuvette holder, temperature-regulated cuvette sample change, and integrating sphere available.

V300b Varian Mercury 300 MHz NMR Spectrometer (Manual) (Currently Offline)
Short Name: V300b
Instrument Type: NMR & EPR
Instrument Location: KCL 3
Date of Acquisition: October 2010

2 channel system, quad nucleus direct-detect probe normally installed.

Best experiments:
 
  • PROTON
  • VT experiments
  • Kinetics experiments
  • P31
  • F19 (including 1H decoupling)
Acceptable experiments (not recommended):
 
  • COSY
  • HSQC/HMBC
  • NOESY 2D
  • C13CPD/C13APT
Not available:
 
  • Nuclei other than H1, C13, F19 & P31
V500wb Varian Inova wide-bore 500 MHz NMR spectrometer (Currently Offline)
Short Name: V500wb
Instrument Type: NMR & EPR
Instrument Location: CRB 100
Date of Acquisition: December 1995

Currently offline for reinstallation.

V600a Varian VNMRS 600 MHz NMR Spectrometer (Manual)
Short Name: V600a
Instrument Type: NMR & EPR
Instrument Location: CRB 100
Date of Acquisition: July 2009

3 channel system. Inverse-detect HCN probe installed.

V600b Varian VNMRS 600 MHz NMR Spectrometer (Manual) (Currently Offline)
Short Name: V600b
Instrument Type: NMR & EPR
Instrument Location: CRB 100
Date of Acquisition: August 2002

3 channel system. Inverse-detect HCN probe installed.

Currently offline for reinstallation.