A400a Agilent DD2 400 MHz NMR Spectrometer (Autosampler)

Short Name: 
A400a
Instrument Type: 
NMR & EPR
Location: 
KCL 3
Date of Acquisition: 
August 2013
Description: 

2 channel system with AS7620 96-sample changer. OneNMR
probe with auto-tune-and-match installed.

Undergraduates have priority on this instrument during lab periods.

Best experiments:

  • PROTON
  • COSY
  • Other nuclei: F19 (no H1 decoupling), P31, B11, (others by request)

Acceptable experiments:

  • C13CPD (night time)
  • 2D HSQC and HMBC experiments
  • 1D/2D NOE experiments

Not available:

  • Variable Temperature experiments
  • Kinetics experiments
  • Simultaneous F19 and H1 experiments

Time Required for NMR Experiments on A400a:

Mass Concentration Proton Carbon gCOSY  gHSQC gHMBC
0.03 mg 0.1 mM 10 h 40 min 35389 hours 1785 hours 1886 hours 7129 hours
0.06 mg 0.2 mM 2 h 40 min 10516 hours 46 h 28 min 474 h 12 min 1785 hours
0.15 mg 0.5 mM 0 h 26 min 1415 h 33 min 6 h 10 min 75 h 01 min 284 h 44 min
0.3 mg 1 mM 0 h 06 min 354 h 01 min 1 h 40 min 18 h 53 min 71 h 19 min
0.6 mg 2 mM 0 h 01 min 88 h 31 min 0 h 26 min 4 h 42 min 17 h 51 min
1.5 mg 5 mM < 1 min 14 h 09 min 0 h 03 min 0 h 45 min 2 h 51 min
3 mg 10 mM < 1 min 3 h 32 min 0 h 03 min 0 h 16 min 1 h 07 min
6 mg 20 mM < 1 min 0 h 53 min 0 h 03 min 0 h 08 min 0 h 17 min
12 mg 40 mM < 1 min 0 h 13 min 0 h 03 min 0 h 08 min 0 h 17 min
15 mg 50 mM < 1 min 0 h 08 min 0 h 03 min 0 h 08 min 0 h 17 min
> 30 mg 100+ mM < 1 min 0 h 04 min 0 h 03 min 0 h 08 min 0 h 17 min

All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube

Instrument Image: