Analytical ultracentrifugation is a column-free separation technique that measures the relative change in the distribution of molecular weights, providing a way to measure heterogeneity, stoichiometry and self-association of proteins, oligomers, aggregates, particles, colloids, and small structures in solution.
Chirascan is a modern, high performance circular dichroism (CD) spectrometer operating in the ultraviolet and visible wavelength range. It is typically used to probe the secondary structure or conformation of bio-molecules.
Dynamic light scattering measures the diffusion rate of molecules or particles in solution by observing the fluctuations in scattered light from a laser passing through a sample. The sensitivity of DLS increases with increasing molecular size, with a typical lower size limit of 0.5 nanometers and upper size limit of 10 micrometers.
Typical applications for DLS include:
- Screening macromolecules prior to crystallization trials
- Measuring the stability of protein samples for NMR analysis
- Monitoring molecular aggregation
- Characterizing nanoparticles
The DynaPro PlateReader II is set up to make these measurements in 96, 384, or 1536 well plates using as little as 5 microliters of sample.
The Microcal VP DSC can characterize the stability of a biomolecule directly in its native form by measuring the heat change associated with its thermal denaturation when heated at a constant rate.
By determining heat changes associated with binding between any two biomolecules, ITC measures all binding parameters in a single label-free, in-solution experiment.
The Florolog 3 is a modular fluorimeter that can be set up to make time-domain fluorescence measurements at specific wavelengths, or steady-state fluorescence measurements over a range of excitation and/or emission wavelengths.